SomaGenics offers library preparation and sequencing services for RNAs, miRNAs and other small RNAs from total RNA (tissue or cell culture) or from biofluid samples. Please enquire for pricing and project discussion.

small RNA library preparation and sequencing services (from total RNA samples)


total RNA samples - from tissue or cells 

This service includes library preparation using RealSeq®-ACplus technology, library QC and sequencing on an Illumina platform (MiniSeq or Next Seq).

Sample quality is essential for successful sequencing results. Our minimum requirement for submitted samples is 5ng/library at a concentration of 1ng/ul. Please enquire if you would like SomaGenics to perform RNA preparation.


total RNA samples - from biofluids

This service includes library preparation using RealSeq®-biofluids technology, library QC and sequencing on an Illumina platform (MiniSeq or Next Seq).

Sample quality is essential for successful sequencing results. Customers are responsible for submitting a high quality RNA preparation. RNA should ideally be isolated from a sample size of 200 ul or more, with a minimum input of 50 ul. Most RNA prepared from biofluids is difficult to quantify and QC, and SomaGenics will prepare the sequencing library and perform sequencing only if the QC of the library is satisfactory. Please note that samples that are preserved in Heparin will not give satisfactory results as Heparin inhibits enzymes in the library preparation steps. 


small RNA library preparation and sequencing services (from biological samples)


This service includes Zymo kit RNA extraction and library preparation using RealSeq®-ACplus technology and library QC and sequencing on an Illumina platform (MiniSeq or Next Seq).

library preparation - from tissue or cells


This service includes RNA extraction from biofluids using Zymo columns, library preparation using RealSeq®-biofluids technology, library QC and sequencing on an Illumina platform (MiniSeq or Next Seq).

The optimal provided sample size is 200ul and the minimum sample size is 50 ul. Please enquire for biofluids other than plasma or serum. Please note that samples that are preserved in Heparin will not give satisfactory results as Heparin inhibits enzymes in the library preparation steps.

library preparation - from biofluids


Bioinformatic analysis

 

Bioinformatic analysis of SomaGenics sequencing results is available. Please enquire for pricing.