Kit components

Reagent

Circularization Reaction Mix

RT Primer

RT Dilution Buffer

Rnase Free Water

PCR Primers

Storage

-20°C

-20°C 

-20°C 

-20°C

-20°C 

Tube

CRM

RP

RTD

W

PCR-P


User-supplied Reagents 

CircLigase II (Epicentre, cat# CL9021K)

RNAseOUT (Thermo Fisher Scientific, cat# 10777019)

Superscript IV Reverse Transcriptase (Thermo Fisher Scientific, cat# 18090010)

SYBR green reagents such as 2X iQ SYBR Green Supermix (BioRad, cat# 170-8880)

Rox reference dye (Thermo Fisher Scientific, cat# 12223012)