RealSeq®-biofluids is a kit for small RNA library preparation that is compatible with Illumina Next-generation sequencing (NGS) technology.  RealSeq®-biofluids is optimized for biofluid samples. The kit accommodates sample volumes of 50ul and up. SomaGenics recommends RNA extraction with Quick-cfRNA columns from Zymo Research.

Kit components

Reagent

RNA buffer 

RealSeq Adapter

RNAse inhibitor

Ligation buffer 

Ligase

Blocking Agent

Blocking Enzyme 

RealSeq Enzyme

RealSeq Buffer 

Dimer Removal Agent

RT Primer

dNTPs

RT buffer

RT Enzyme

PCR Buffer

PCR Polymerase

Rnase Free Water

HY4 Removal Agent

RealSeq Beads

Reverse Index Primers*

Forward Primer

miRNA Control

SPRIselect Reagent

Storage

-20°C

-20°C 

-20°C 

-20°C

-20°C 

-20°C 

-20°C

-20°C 

-20°C

-20°C 

-20°C 

-20°C

-20°C 

-20°C 

-20°C

-20°C 

-20°C 

-20°C

-20°C

-20°C 

-20°C 

-20°C 

4° to 25° C


* Index Primers 1-12 are included in 600-00012 and 600-00024 (12- and 24-reaction kits) and Index Primers 1-24 are included in 600-00048 (48-reaction kit).  Additional Index Primers can be purchased separately.