RealSeq®-biofluids is a kit for small RNA library preparation that is compatible with Illumina Next-generation sequencing (NGS) technology. RealSeq®-biofluids is optimized for biofluid samples. The kit accommodates sample volumes of 50ul and up. SomaGenics recommends RNA extraction with Quick-cfRNA columns from Zymo Research.
Kit components
Reagent
RNA buffer
RealSeq Adapter
RNAse inhibitor
Ligation buffer
Ligase
Blocking Agent
Blocking Enzyme
RealSeq Enzyme
RealSeq Buffer
Dimer Removal Agent
RT Primer
dNTPs
RT buffer
RT Enzyme
PCR Buffer
PCR Polymerase
Rnase Free Water
HY4 Removal Agent
RealSeq Beads
Reverse Index Primers*
Forward Primer
miRNA Control
SPRIselect Reagent
Storage
-20°C
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4° to 25° C
* Index Primers 1-12 are included in 600-00012 and 600-00024 (12- and 24-reaction kits) and Index Primers 1-48 are included in 600-00048 (48-reaction kit). Additional Index Primers can be purchased separately.