RealSeq®-AC is a kit for small RNA library preparation that is compatible with Illumina Next-generation sequencing (NGS) technology. RealSeq®-AC is optimized for inputs between 1 ng and 1000 ng of total RNA from tissue or cells.

Kit components

Reagent

RNA buffer 

RealSeq Adapter

RNAse inhibitor

Ligation buffer 

Ligase

Blocking Agent

Blocking Enzyme

Blocking Buffer 

RealSeq Enzyme

RealSeq Buffer 

Dimer Removal Agent

RT Primer

dNTPs

RT buffer

RT Enzyme

PCR Buffer

PCR Polymerase

Rnase Free Water

RealSeq Beads

Reverse Index Primers*

Forward Primer

miRNA Control

Adapter Dilution Buffer

SPRIselect reagent

Storage

-20°C

-20°C 

-20°C 

-20°C

-20°C 

-20°C 

-20°C

-20°C 

-20°C 

-20°C

-20°C 

-20°C 

-20°C

-20°C 

-20°C 

-20°C

-20°C 

-20°C 

-20°C

-20°C 

-20°C 

-20°C

-20°C 

4° to 25° C


* Index Primers 1-12 are included in 500-00012 and 500-00024 (12- and 24-reaction kits) and Index Primers 1-24 are included in 500-00048 (48-reaction kit).  Additional Index Primers can be purchased separately.