RealSeq®-AC is a kit for small RNA library preparation that is compatible with Illumina Next-generation sequencing (NGS) technology. RealSeq®-AC is optimized for inputs between 1 ng and 1000 ng of total RNA from tissue or cells.
Kit components
Reagent
RNA buffer
RealSeq Adapter
RNAse inhibitor
Ligation buffer
Ligase
Blocking Agent
Blocking Enzyme
Blocking Buffer
RealSeq Enzyme
RealSeq Buffer
Dimer Removal Agent
RT Primer
dNTPs
RT buffer
RT Enzyme
PCR Buffer
PCR Polymerase
Rnase Free Water
RealSeq Beads
Reverse Index Primers*
Forward Primer
miRNA Control
Adapter Dilution Buffer
Storage
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* Index primers 1-12 are included in 500-00012 (12-reaction kit), index primers 1-24 are included in 500-00024 (24-reaction kit), and Index Primers 1-48 are included in 500-00048 (48-reaction kit). Additional Index Primers can be purchased separately.